Accelerate your microbiome discovery with our microbial isolation and cultivation service

Access the full power of the Prospector® and our dedicated team of scientists to rapidly generate microbial isolates for you.
Increase discoveries, skip the Petri dishes

How Isolation Bio’s cultivation service works:

It is as simple as sending samples in and receiving research ready isolates back. Our workflow is optimized to yield a high number of isolates from fecal and soil samples.

Step 01

Form

Fill out contact information and briefly describe your sample type

Step 02

Consultation

Complete consultation call with an Isolation Bio scientist to discuss your samples and experiment needs

Step 03

Samples

Send us your samples

Step 04

Prospector®

We cultivate your microbes on the Prospector. The Prospector has been validated for a multitude of aerobic and anaerobic media types but is also compatible with additional types not listed.

Step 05

96-Well Plate

Isolates are transferred to 96-well plates and shipped back to you as glycerol stocks

The power of the Prospector® discovery platform

The Prospector® enables:

Isolate, cultivate, and screen the living microbiome thousands of microbes at a time.

Our high-throughput and scalable microbial cultivation array technology grows thousands of microcolonies in parallel – delivering more unique isolates in less time than traditional methods.

The power of the Prospector® discovery platform

The Prospector® enables:

Isolate, cultivate, and screen the living microbiome thousands of microbes at a time.

Our high-throughput and scalable microbial cultivation array technology grows thousands of microcolonies in parallel – delivering more unique isolates in less time than traditional methods..

Frequently asked questions

Yes. Provide 500 mL (volume may differ based on project goals) in two or more bottles. Media must be sterile. Indicate pH and composition. We will internally validate the media before using in project. Pack very well when shipping to prevent contamination, breakage and spillage.​

To date, only LMS and LMRS are fully incompatible. Low and high pH are case-dependent and will require validation. Media below pH 6.5 and above pH 9 are less likely to be compatible. ​

Yes, but case dependent. ​

We can load the Prospector arrays with a more general medium (e.g., BHI) and then transfer from the arrays to plates that have the selective medium (e.g., LMRS). The number of successful transfers will be low, but what does transfer will likely be your target phenotype.​

Array growth is monitored via fluorescent signal and the color of the medium does not matter. Very dark media, such as those with high percentages of rumen fluid, cannot be used in the downstream transfer plates since we reply on visible turbidity to identify growth. ​

Yes. Provide enrichment details and any specialized reagents (e.g., sporulation factors). Enrichment will skew the community towards phenotype being enriched.​

Use a larger volume of inoculum of thawed and mixed freezer stock (e.g., 20 uL), spread on blood agar plates (YCFAC-blood or BHI-blood), incubate for longer period of time (one week or longer). If you know the identify of the isolate, revive under media/incubation conditions optimal for that species.​

Contact us to discuss your microbiome program